Hotspot

trnK-AAA-rps16

LSC · 4337-5020 · 684 bp

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Pi 0.0186

MarkerSeek score 62.70

Primer available yes

Label trnK-AAA-rps16

Feature Metadata

label_name: trnK-AAA-rps16
feature_type: igs
region: LSC
length_bp: 684
variable_sites: 26
pi: 0.0186
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-AAA-rps16_p1	CTACCGCAGATTTGGACGAA	CCCAATGAGCCGTTTATCGA	50.0	50.0	58.0	58.1	976-1036 bp	1.0	85.1
trnK-AAA-rps16_p2	CTACCGCAGATTTGGACGAA	TCCCAATGAGCCGTTTATCG	50.0	50.0	58.0	58.1	977-1037 bp	1.0	85.1
trnK-AAA-rps16_p3	CCTTTCAGGATCAGTCGTGG	CCCAATGAGCCGTTTATCGA	55.0	50.0	58.0	58.1	1007-1067 bp	1.0	84.9
trnK-AAA-rps16_p4	CTACCGCAGATTTGGACGAA	GGTTTGTTGCTGCCCTTTTT	50.0	45.0	58.0	58.0	1463-1514 bp	1.0	84.1
trnK-AAA-rps16_p5	CCTTTCAGGATCAGTCGTGG	GGTTTGTTGCTGCCCTTTTT	55.0	45.0	58.0	58.0	1494-1545 bp	1.0	83.9