Hotspot

ycf4

LSC · 64292-64846 · 555 bp

Back to Bobgunnia

Pi 0.0036

MarkerSeek score 59.90

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: LSC
length_bp: 555
variable_sites: 2
pi: 0.0036
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	AGCGCTAGTTGATGAAAGTT	AACCAAAGAACGCCCTTTCT	40.0	45.0	55.4	57.9	757 bp	1.0	58.8
ycf4_p2	AGCGCTAGTTGATGAAAGTT	TGAAACCAAAGAACGCCCTT	40.0	45.0	55.4	58.2	760 bp	1.0	57.5
ycf4_p3	AGCGCTAGTTGATGAAAGTT	TTGAAACCAAAGAACGCCCT	40.0	45.0	55.4	58.2	761 bp	1.0	57.5
ycf4_p4	AGCGCTAGTTGATGAAAGTT	TTTGAAACCAAAGAACGCCC	40.0	45.0	55.4	57.4	762 bp	1.0	54.7
ycf4_p5	AGTCAAATTCATTTGGTTGGGT	AACCAAAGAACGCCCTTTCT	36.4	45.0	56.7	57.9	714 bp	1.0	53.3