Hotspot

rpl16

LSC · 86648-88215 · 1568 bp

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Pi 0

MarkerSeek score 59.70

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1568
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	AGACCCCTTTCCTTTGGTTC	CCATTCTTATTCTGTTAAATCAAAACA	50.0	25.9	57.3	55.5	1679-1697 bp	1.0	40.9
rpl16_p2	GACCCCTTTCCTTTGGTTCT	CCATTCTTATTCTGTTAAATCAAAACA	50.0	25.9	57.3	55.5	1678-1696 bp	1.0	40.9
rpl16_p3	AGACCCCTTTCCTTTGGTTC	TCCATTCTTATTCTGTTAAATCAA	50.0	25.0	57.3	52.4	1680-1698 bp	1.0	40.9
rpl16_p4	GACCCCTTTCCTTTGGTTCT	TCCATTCTTATTCTGTTAAATCAA	50.0	25.0	57.3	52.4	1679-1697 bp	1.0	40.9
rpl16_p5	GACCCCTTTCCTTTGGTTCT	TTCCATTCTTATTCTGTTAAATCA	50.0	25.0	57.3	52.4	1680-1698 bp	1.0	40.9