Hotspot

rpl33-rps18

LSC · 72307-72525 · 219 bp

Back to Bobgunnia

Pi 0.00995

MarkerSeek score 62.10

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 219
variable_sites: 2
pi: 0.00995
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CGACACAATACGCCTAGTCG	TCAATTCGATCCCCCGATTG	55.0	50.0	58.5	57.7	374-392 bp	1.0	77.9
rpl33-rps18_p2	AATTCTGTCCCTGCTGTTGC	TCAATTCGATCCCCCGATTG	50.0	50.0	58.7	57.7	340-358 bp	1.0	76.4
rpl33-rps18_p3	GCGAAAATTCTGTCCCTGCT	TCAATTCGATCCCCCGATTG	50.0	50.0	58.8	57.7	345-363 bp	1.0	75.8
rpl33-rps18_p4	GACACAATACGCCTAGTCGA	TCAATTCGATCCCCCGATTG	50.0	50.0	57.2	57.7	373-391 bp	1.0	75.4
rpl33-rps18_p5	TCGACACAATACGCCTAGTC	TCAATTCGATCCCCCGATTG	50.0	50.0	57.2	57.7	375-393 bp	1.0	75.4