Hotspot

psbZ-trnG-UCC

LSC · 39092-39893 · 802 bp

Back to Bobgunnia

Pi 0.00506

MarkerSeek score 53.60

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 802
variable_sites: 4
pi: 0.00506
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	959-980 bp	1.0	78.2
psbZ-trnG-UCC_p2	GCTTCTCCTGATGGTTGGTC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.3	57.8	957-978 bp	1.0	77.8
psbZ-trnG-UCC_p3	CTCCTGATGGTTGGTCAAGT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.1	57.8	953-974 bp	1.0	72.9
psbZ-trnG-UCC_p4	TGCTTCTCCTGATGGTTGGT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.9	57.8	958-979 bp	1.0	72.4
psbZ-trnG-UCC_p5	TCTCCTGATGGTTGGTCAAG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.8	57.8	954-975 bp	1.0	70.6