Hotspot

rps16-trnQ-UUG

LSC · 6436-7250 · 815 bp

Back to Bobgunnia

Pi 0.0105
MarkerSeek score 69.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
815
variable_sites
8
pi
0.0105
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TCTTTCAAGTCACACGTTGC GAGGTTCGAATCCTTCCGTC 45.0 55.0 56.9 58.1 839-894 bp 1.0 75.3
rps16-trnQ-UUG_p2 CTTTCAAGTCACACGTTGCT GAGGTTCGAATCCTTCCGTC 45.0 55.0 56.9 58.1 838-893 bp 1.0 75.2
rps16-trnQ-UUG_p3 CACACGTTGCTTTTTACCACA GAGGTTCGAATCCTTCCGTC 42.9 55.0 57.9 58.1 829-884 bp 1.0 75.0
rps16-trnQ-UUG_p4 ACGTTGCTTTTTACCACATCG GAGGTTCGAATCCTTCCGTC 42.9 55.0 57.7 58.1 826-881 bp 1.0 74.0
rps16-trnQ-UUG_p5 CGTTGCTTTTTACCACATCGT GAGGTTCGAATCCTTCCGTC 42.9 55.0 57.7 58.1 825-880 bp 1.0 74.0