Hotspot

psbA

LSC · 262-1323 · 1062 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label psbA

Feature Metadata

label_name
psbA
feature_type
gene
region
LSC
length_bp
1062
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA_p1 TCTATTCTACTTCAACAATTCGT GTGCTTGGGAGTCCTTGAAA 30.4 50.0 53.6 58.0 1146 bp 1.0 40.0
psbA_p2 TTCTATTCTACTTCAACAATTCGT GTGCTTGGGAGTCCTTGAAA 29.2 50.0 54.3 58.0 1147 bp 1.0 40.0
psbA_p3 TCTATTCTACTTCAACAATTCGT TATGTGTGCTTGGGAGTCCT 30.4 50.0 53.6 58.3 1151 bp 1.0 40.0
psbA_p4 AATTCGACCTTATTGAATCAAA GTGCTTGGGAGTCCTTGAAA 27.3 50.0 52.1 58.0 1215 bp 1.0 40.0
psbA_p5 TTTCTATTCTACTTCAACAATTCGT GTGCTTGGGAGTCCTTGAAA 28.0 50.0 55.1 58.0 1148 bp 1.0 40.0