Hotspot

atpA

LSC · 10499-12022 · 1524 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label atpA

Feature Metadata

label_name
atpA
feature_type
gene
region
LSC
length_bp
1524
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA_p1 AGACATTCATCAACAACAACTCT ATTGGCATCCTAGGGACCAT 34.8 50.0 56.4 58.2 1722 bp 1.0 41.8
atpA_p2 AAGACATTCATCAACAACAACTCT ATTGGCATCCTAGGGACCAT 33.3 50.0 57.1 58.2 1723 bp 1.0 40.0
atpA_p3 AGACATTCATCAACAACAACTCTT ATTGGCATCCTAGGGACCAT 33.3 50.0 57.1 58.2 1722 bp 1.0 40.0
atpA_p4 AGACATTCATCAACAACAACTCT TATTGGCATCCTAGGGACCA 34.8 50.0 56.4 57.5 1723 bp 1.0 40.0
atpA_p5 GACATTCATCAACAACAACTCT ATTGGCATCCTAGGGACCAT 36.4 50.0 55.0 58.2 1721 bp 1.0 40.0