Hotspot

trnS-GCU-trnG-UCC

LSC · 8388-9318 · 931 bp

Back to Blyxa

Pi 0
MarkerSeek score 40.00
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
931
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 1000 bp 1.0 51.7
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 999 bp 1.0 51.7
trnS-GCU-trnG-UCC_p3 CTCAAGCGATCATAGGGAGC ACGAATCACACTTTTACCACT 55.0 38.1 57.9 55.5 1072 bp 1.0 51.1
trnS-GCU-trnG-UCC_p4 CAATCCGACGCTTTAGTCCA AGAACGAATCACACTTTTACCAC 50.0 39.1 58.0 57.3 1003 bp 1.0 50.6
trnS-GCU-trnG-UCC_p5 CAATCCGACGCTTTAGTCCA GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 1002 bp 1.0 50.6