Hotspot

trnQ-UUG-psbK

LSC · 6806-7455 · 650 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label trnQ-UUG-psbK

Feature Metadata

label_name
trnQ-UUG-psbK
feature_type
igs
region
LSC
length_bp
650
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-psbK_p1 ACCTCCGAATAACAGGACCA GGCAATTTGGCGAAGAAAAA 50.0 40.0 58.1 56.0 783 bp 1.0 63.7
trnQ-UUG-psbK_p2 GACCAAAACCCGTTGCCTTA GGCAATTTGGCGAAGAAAAA 50.0 40.0 58.7 56.0 768 bp 1.0 58.7
trnQ-UUG-psbK_p3 TAACAGGACCAAAACCCGTT GGCAATTTGGCGAAGAAAAA 45.0 40.0 57.3 56.0 774 bp 1.0 58.2
trnQ-UUG-psbK_p4 AACAGGACCAAAACCCGTTG GGCAATTTGGCGAAGAAAAA 50.0 40.0 58.9 56.0 773 bp 1.0 57.0
trnQ-UUG-psbK_p5 CGAACCTCCGAATAACAGGA GGCAATTTGGCGAAGAAAAA 50.0 40.0 57.0 56.0 786 bp 1.0 56.5