Hotspot

ycf1

SSC · 121794-126902 · 5109 bp

Back to Blumea

Pi 0.0137
MarkerSeek score 45.20
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5109
variable_sites
181
pi
0.0137
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 GACGGGCAGGTTCCATAATT AATCGCCGCATATCGAATCC 50.0 50.0 57.9 58.6 514 bp 0.5 55.9
ycf1_p2 ATAGACGGGCAGGTTCCATA AATCGCCGCATATCGAATCC 50.0 50.0 57.9 58.6 517 bp 0.5 55.5
ycf1_p3 TAGACGGGCAGGTTCCATAA AATCGCCGCATATCGAATCC 50.0 50.0 58.1 58.6 516 bp 0.5 55.2
ycf1_p4 TACAGATAGACGGGCAGGTT AATCGCCGCATATCGAATCC 50.0 50.0 57.8 58.6 522 bp 0.5 55.0
ycf1_p5 TTACAGATAGACGGGCAGGT AATCGCCGCATATCGAATCC 50.0 50.0 57.8 58.6 523 bp 0.5 55.0