Hotspot

trnL-CAA-ycf2

IRa · 141819-142233 · 415 bp

Back to Blumea

Pi 0.0203
MarkerSeek score 59.80
Primer available yes
Label trnL-CAA-ycf2

Feature Metadata

label_name
trnL-CAA-ycf2
feature_type
igs
region
IRa
length_bp
415
variable_sites
17
pi
0.0203
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-CAA-ycf2_p1 AAATGGTAGACACGCGAGAC GCTTTTCCCGGATGAAATGC 50.0 50.0 58.0 58.1 569 bp 1.0 81.7
trnL-CAA-ycf2_p2 TGAAATGGTAGACACGCGAG GCTTTTCCCGGATGAAATGC 50.0 50.0 58.0 58.1 571 bp 1.0 81.7
trnL-CAA-ycf2_p3 GCCTTGGTGGTGAAATGGTA GCTTTTCCCGGATGAAATGC 50.0 50.0 58.1 58.1 581 bp 1.0 81.1
trnL-CAA-ycf2_p4 AAATGGTAGACACGCGAGAC ATGGCTTTTCCCGGATGAAA 50.0 45.0 58.0 57.8 572 bp 1.0 80.5
trnL-CAA-ycf2_p5 AAATGGTAGACACGCGAGAC TGGCTTTTCCCGGATGAAAT 50.0 45.0 58.0 57.8 571 bp 1.0 80.5