Hotspot

ccsA-ndhD

SSC · 113325-113590 · 266 bp

Back to Blumea

Pi 0.0367
MarkerSeek score 63.80
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
266
variable_sites
23
pi
0.0367
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 CGGCTCTTCTTTGTGGATCA GTTTTTGATTCTGGACCGCG 50.0 50.0 57.9 58.0 923-930 bp 1.0 82.4
ccsA-ndhD_p2 CGGCTCTTCTTTGTGGATCA CTCCCGGCCAACGTAATTTA 50.0 50.0 57.9 58.0 1399-1406 bp 1.0 81.9
ccsA-ndhD_p3 CGGCTCTTCTTTGTGGATCA CATGGCTTTATTGGTGCTGC 50.0 50.0 57.9 58.1 1271-1278 bp 1.0 81.8
ccsA-ndhD_p4 TCAGGAGCAGTATGGGCTAA CTCCCGGCCAACGTAATTTA 50.0 50.0 57.8 58.0 1133-1140 bp 1.0 81.5
ccsA-ndhD_p5 TTCAGGAGCAGTATGGGCTA CTCCCGGCCAACGTAATTTA 50.0 50.0 57.8 58.0 1134-1141 bp 1.0 81.5