Hotspot

rpl20-rps12

LSC · 67561-68295 · 735 bp

Back to Blumea

Pi 0.0125
MarkerSeek score 62.60
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
735
variable_sites
24
pi
0.0125
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 TCTCCGAGCGATATATCCCC CAGCGTCGAGGAACATGTAC 55.0 55.0 57.9 58.7 794-801 bp 1.0 77.2
rpl20-rps12_p2 TCTCCGAGCGATATATCCCC AGCGTCGAGGAACATGTACT 55.0 50.0 57.9 58.8 793-800 bp 1.0 76.5
rpl20-rps12_p3 TCTCCGAGCGATATATCCCC TCAGCGTCGAGGAACATGTA 55.0 50.0 57.9 58.8 795-802 bp 1.0 76.4
rpl20-rps12_p4 TCTCCGAGCGATATATCCCC GCGTCGAGGAACATGTACTA 55.0 50.0 57.9 57.2 792-799 bp 1.0 76.4
rpl20-rps12_p5 TCTCCGAGCGATATATCCCC ATGTCACAAAATCCCCTGCG 55.0 50.0 57.9 58.8 829-836 bp 1.0 76.3