Hotspot

psbM-trnD-GUC

LSC · 10952-11622 · 671 bp

Back to Blumea

Pi 0.0158
MarkerSeek score 61.00
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
671
variable_sites
25
pi
0.0158
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.94
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGTAGAAAGGCAGTAGGAACT TTCAATAGGCAAGAGCACCG 42.9 50.0 55.7 58.3 764-803 bp 1.0 58.2
psbM-trnD-GUC_p2 GGAACTAAAATGAACAGTGCAGT TTCAATAGGCAAGAGCACCG 39.1 50.0 57.6 58.3 749-788 bp 1.0 57.0
psbM-trnD-GUC_p3 AAAATGAACAGTGCAGTAGC TTCAATAGGCAAGAGCACCG 40.0 50.0 54.5 58.3 743-782 bp 1.0 56.6
psbM-trnD-GUC_p4 AAGTAGAAAGGCAGTAGGAACT TTCAATAGGCAAGAGCACCG 40.9 50.0 56.5 58.3 765-804 bp 1.0 56.3
psbM-trnD-GUC_p5 ACTAAAATGAACAGTGCAGTAGC TTCAATAGGCAAGAGCACCG 39.1 50.0 57.4 58.3 746-785 bp 1.0 55.6