Hotspot

ycf1

SSC · 123351-128885 · 5535 bp

Back to Blitum

Pi 0.0158
MarkerSeek score 47.50
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5535
variable_sites
87
pi
0.0158
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TCTGATTGAATGTTGATTCCCC AAATGGGTCCTATCGTGGTA 40.9 45.0 56.4 55.6 227-248 bp 1.0 46.7
ycf1_p2 TCTGATTGAATGTTGATTCCCC TAAATGGGTCCTATCGTGGT 40.9 45.0 56.4 55.6 228-249 bp 1.0 46.7
ycf1_p3 TCTGATTGAATGTTGATTCCCC TTAAATGGGTCCTATCGTGGT 40.9 42.9 56.4 56.4 229-250 bp 1.0 46.7
ycf1_p4 TCTGATTGAATGTTGATTCCCC AATGGGTCCTATCGTGGTAT 40.9 45.0 56.4 55.4 226-247 bp 1.0 46.7
ycf1_p5 TTCTGATTGAATGTTGATTCCCC AAATGGGTCCTATCGTGGTA 39.1 45.0 57.1 55.6 228-249 bp 1.0 46.7