Hotspot

rpl22

LSC · 83473-84072 · 600 bp

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Pi 0.0233
MarkerSeek score 58.30
Primer available yes
Label rpl22

Feature Metadata

label_name
rpl22
feature_type
gene
region
LSC
length_bp
600
variable_sites
14
pi
0.0233
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22_p1 CAAGTCGGAAACCAAGTGGA TGGGTAGAGCCGGATCTAAG 50.0 55.0 58.0 58.0 1272-1273 bp 1.0 80.9
rpl22_p2 ATCGATACGGCCTGCAATTT TGGGTAGAGCCGGATCTAAG 45.0 55.0 58.0 58.0 1742-1743 bp 1.0 80.8
rpl22_p3 TTTTCCATCGATACGGCCTG TGGGTAGAGCCGGATCTAAG 50.0 55.0 58.1 58.0 1748-1749 bp 1.0 80.7
rpl22_p4 AGGGTGCCCATAAGGTTTTG TGGGTAGAGCCGGATCTAAG 50.0 55.0 58.1 58.0 1607-1608 bp 1.0 80.6
rpl22_p5 ATACGGCCTGCAATTTGGAT TGGGTAGAGCCGGATCTAAG 45.0 55.0 57.9 58.0 1738-1739 bp 1.0 80.5