Hotspot

rrn4.5-rrn23

IRa · 130682-130742 · 61 bp

Back to Blitum

Pi 0.0656
MarkerSeek score 73.80
Primer available yes
Label rrn4.5-rrn23

Feature Metadata

label_name
rrn4.5-rrn23
feature_type
igs
region
IRa
length_bp
61
variable_sites
4
pi
0.0656
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn4.5-rrn23_p1 TCACTGCACTTCCACTTGAC AAGTAGTAAGCCCACCCCAA 50.0 50.0 58.0 58.2 157-160 bp 1.0 79.6
rrn4.5-rrn23_p2 ATGATAAACGGCTCGTCTCG AAGTAGTAAGCCCACCCCAA 50.0 50.0 57.9 58.2 127-130 bp 1.0 79.5
rrn4.5-rrn23_p3 TCACTGCACTTCCACTTGAC TAGTAAGCCCACCCCAAGAT 50.0 50.0 58.0 57.7 154-157 bp 1.0 79.4
rrn4.5-rrn23_p4 TCACTGCACTTCCACTTGAC GTACCAGTTATCGTGCCCAC 50.0 55.0 58.0 58.4 227-230 bp 1.0 78.2
rrn4.5-rrn23_p5 TCACTGCACTTCCACTTGAC GTGTACCAGTTATCGTGCCC 50.0 55.0 58.0 58.4 229-232 bp 1.0 78.2