Hotspot

rpl32-trnL-UAG

SSC · 112664-113745 · 1082 bp

Back to Blitum

Pi 0.0355
MarkerSeek score 78.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1082
variable_sites
30
pi
0.0355
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.78
markerseek_score
78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGATATTGGGCGGCGTTAAA CTCGCACTGCTTCCTAAGAG 50.0 55.0 58.7 58.1 985-1216 bp 1.0 80.0
rpl32-trnL-UAG_p2 GGATATTGGGCGGCGTTAAA CTCTCGCACTGCTTCCTAAG 50.0 55.0 58.7 58.1 987-1218 bp 1.0 80.0
rpl32-trnL-UAG_p3 GGATATTGGGCGGCGTTAAA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.7 57.9 974-1205 bp 1.0 79.7
rpl32-trnL-UAG_p4 GGATATTGGGCGGCGTTAAA AGCAGCGTGTCTACCAATTT 50.0 45.0 58.7 57.8 967-1198 bp 1.0 79.2
rpl32-trnL-UAG_p5 GGATATTGGGCGGCGTTAAA CTAAGAGCAGCGTGTCTACC 50.0 55.0 58.7 57.8 972-1203 bp 1.0 79.1