Hotspot

psbE-petL

LSC · 64613-65758 · 1146 bp

Back to Blitum

Pi 0.0255
MarkerSeek score 67.50
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1146
variable_sites
29
pi
0.0255
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA GCGGAAGTTATAGTTAAAGCTGC 50.0 43.5 57.9 58.2 1309-1329 bp 1.0 56.5
psbE-petL_p2 AGAACGTTCTCCTGTGCTTC GCGGAAGTTATAGTTAAAGCTGC 50.0 43.5 57.8 58.2 1232-1252 bp 1.0 55.8
psbE-petL_p3 ACGAATAACCAACCCGCAAT GCGGAAGTTATAGTTAAAGCTGC 45.0 43.5 58.2 58.2 1318-1338 bp 1.0 55.5
psbE-petL_p4 GTTCTCCTGTGCTTCCAGAC GCGGAAGTTATAGTTAAAGCTGC 55.0 43.5 58.2 58.2 1227-1247 bp 1.0 55.5
psbE-petL_p5 CGAATAACCAACCCGCAATG GCGGAAGTTATAGTTAAAGCTGC 50.0 43.5 57.8 58.2 1317-1337 bp 1.0 55.4