Hotspot

cemA-petA

LSC · 61754-62020 · 267 bp

Back to Blitum

Pi 0.0449
MarkerSeek score 70.70
Primer available yes
Label cemA-petA

Feature Metadata

label_name
cemA-petA
feature_type
igs
region
LSC
length_bp
267
variable_sites
12
pi
0.0449
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
cemA-petA_p1 AAATCGTGTATCCCCATCGC TGGAAATGGATCGAGTAATCTGT 50.0 39.1 58.1 57.4 377-378 bp 1.0 52.7
cemA-petA_p2 AAATCGTGTATCCCCATCGC TGAGTGATATGGAAATGGATCGA 50.0 39.1 58.1 57.2 386-387 bp 1.0 51.1
cemA-petA_p3 AAATCGTGTATCCCCATCGC AGTGATATGGAAATGGATCGAGT 50.0 39.1 58.1 57.2 384-385 bp 1.0 51.0
cemA-petA_p4 CCCCATCGCTTGTAGTGATT TGGAAATGGATCGAGTAATCTGT 50.0 39.1 57.7 57.4 366-367 bp 1.0 51.0
cemA-petA_p5 TCCCCATCGCTTGTAGTGAT TGGAAATGGATCGAGTAATCTGT 50.0 39.1 58.5 57.4 367-368 bp 1.0 49.7