Hotspot

rps4-trnT-UGU

LSC · 45840-46258 · 419 bp

Back to Blitum

Pi 0.0296
MarkerSeek score 68.70
Primer available yes
Label rps4-trnT-UGU

Feature Metadata

label_name
rps4-trnT-UGU
feature_type
igs
region
LSC
length_bp
419
variable_sites
12
pi
0.0296
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-UGU_p1 AGTCCAGGTAAAGCCCCTAG CTCAGAGGTTAGAGCATCGC 55.0 55.0 58.1 57.9 542-544 bp 1.0 81.7
rps4-trnT-UGU_p2 GTCCAGGTAAAGCCCCTAGA CTCAGAGGTTAGAGCATCGC 55.0 55.0 58.1 57.9 541-543 bp 1.0 81.6
rps4-trnT-UGU_p3 TCCAGGTAAAGCCCCTAGAC CTCAGAGGTTAGAGCATCGC 55.0 55.0 58.1 57.9 540-542 bp 1.0 81.6
rps4-trnT-UGU_p4 AGTCCAGGTAAAGCCCCTAG ATGGTCATCGGTTCGAATCC 55.0 50.0 58.1 57.8 510-512 bp 1.0 81.0
rps4-trnT-UGU_p5 TCCAGGTAAAGCCCCTAGAC ATGGTCATCGGTTCGAATCC 55.0 50.0 58.1 57.8 508-510 bp 1.0 81.0