Hotspot

trnT-GGU-psbD

LSC · 30979-32214 · 1236 bp

Back to Blitum

Pi 0.0187
MarkerSeek score 59.20
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1236
variable_sites
23
pi
0.0187
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GGCGTAAGTCATCGGTTCAA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.3 58.2 1363-1375 bp 1.0 77.5
trnT-GGU-psbD_p2 GGCGTAAGTCATCGGTTCAA CAAAAACGAAACGGTCCCTC 50.0 50.0 58.3 57.6 1362-1374 bp 1.0 76.3
trnT-GGU-psbD_p3 GGCGTAAGTCATCGGTTCAA TCCCTCCGTAACCAGTCATC 50.0 55.0 58.3 58.5 1348-1360 bp 1.0 75.4
trnT-GGU-psbD_p4 GCCATGGTAAGGCGTAAGTC ACAAAAACGAAACGGTCCCT 55.0 45.0 58.7 58.2 1373-1385 bp 1.0 74.1
trnT-GGU-psbD_p5 GGCGTAAGTCATCGGTTCAA AAAAACGAAACGGTCCCTCC 50.0 50.0 58.3 58.7 1361-1373 bp 1.0 74.0