Hotspot

trnS-GCU-trnG-UCC

LSC · 7689-8325 · 637 bp

Back to Blitum

Pi 0.0243
MarkerSeek score 70.80
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
637
variable_sites
15
pi
0.0243
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 ACGGAAAGAGAGGGATTCGA AATCAAACCGTTAGACCCCT 50.0 45.0 58.2 56.4 789-803 bp 1.0 69.9
trnS-GCU-trnG-UCC_p2 ACGGAAAGAGAGGGATTCGA ATCAAACCGTTAGACCCCTT 50.0 45.0 58.2 56.4 788-802 bp 1.0 69.9
trnS-GCU-trnG-UCC_p3 CGAATGACTCGTACAACGGA AATCAAACCGTTAGACCCCT 50.0 45.0 57.7 56.4 759-773 bp 1.0 69.2
trnS-GCU-trnG-UCC_p4 CGAATGACTCGTACAACGGA ATCAAACCGTTAGACCCCTT 50.0 45.0 57.7 56.4 758-772 bp 1.0 69.2
trnS-GCU-trnG-UCC_p5 TGGAAACGGAAAGAGAGGGA ATCAAACCGTTAGACCCCTT 50.0 45.0 58.3 56.4 793-807 bp 1.0 68.9