Hotspot

ndhF-rpl32

SSC · 115739-116678 · 940 bp

Back to Bletilla

Pi 0.115
MarkerSeek score 76.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
940
variable_sites
144
pi
0.115
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.59
markerseek_score
76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGTATTATCCATGCATATTTATATGT GCTTTTACTGCCTCTAAAGATCC 26.9 43.5 53.5 57.3 631-1107 bp 1.0 52.0
ndhF-rpl32_p2 TCCATGCATATTTATATGTATATTCCA GCTTTTACTGCCTCTAAAGATCC 25.9 43.5 54.8 57.3 793-1100 bp 0.75 42.0
ndhF-rpl32_p3 TCCATGCATATTTATATGTATATTCCA AGAAAAAGCTTTTACTGCCTCT 25.9 36.4 54.8 56.2 800-1107 bp 0.75 42.0
ndhF-rpl32_p4 CCATGCATATTTATATGTATATTCCA GCTTTTACTGCCTCTAAAGATCC 26.9 43.5 53.5 57.3 792-1099 bp 0.75 42.0
ndhF-rpl32_p5 TCCATGCATATTTATATGTATATTCCA AAGAAAAAGCTTTTACTGCCTCT 25.9 34.8 54.8 56.9 801-1108 bp 0.75 42.0