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clpP-psbB

LSC · 73848-74665 · 818 bp

Back to Bletilla

Pi 0.102

MarkerSeek score 80.30

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 818
variable_sites: 125
pi: 0.102
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	TATACGTCAACCCAAGCTGC	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	724-909 bp	1.0	90.0
clpP-psbB_p2	CTTTTGGAACACCAATGGGC	CGATACCAAGGCAAACCCAT	50.0	50.0	57.8	58.2	675-860 bp	1.0	88.8
clpP-psbB_p3	TATACGTCAACCCAAGCTGC	ACACGATACCAAGGCAAACC	50.0	50.0	58.0	58.5	727-912 bp	1.0	88.1
clpP-psbB_p4	TTTGGAACACCAATGGGCAT	CGATACCAAGGCAAACCCAT	45.0	50.0	58.3	58.2	673-858 bp	1.0	87.9
clpP-psbB_p5	TCCAGGACTCCGAAAAGGTA	CGATACCAAGGCAAACCCAT	50.0	50.0	57.7	58.2	695-880 bp	1.0	87.6