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psaJ-rpl33

LSC · 68718-69299 · 582 bp

Back to Bletilla

Pi 0.0578

MarkerSeek score 83.80

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 582
variable_sites: 63
pi: 0.0578
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GCGGCAAAATTCAATCTGCT	AATGCCCGGCGATTCTTTAT	45.0	45.0	58.0	58.0	1037-1066 bp	0.75	77.0
psaJ-rpl33_p2	GCGGCAAAATTCAATCTGCT	ATGCCCGGCGATTCTTTATT	45.0	45.0	58.0	58.0	1036-1065 bp	0.75	77.0
psaJ-rpl33_p3	GCGGCAAAATTCAATCTGCT	TAATCGATTCGATCCCCCGA	45.0	50.0	58.0	58.1	1429-1453 bp	0.75	75.4
psaJ-rpl33_p4	GCGGCAAAATTCAATCTGCT	GGTCAACCTATTCGTCCGTC	45.0	55.0	58.0	58.1	1505-1529 bp	0.75	75.3
psaJ-rpl33_p5	GCGGCAAAATTCAATCTGCT	GATCGACTCCACTCTCTCCA	45.0	55.0	58.0	58.0	1610-1634 bp	0.75	75.2