Hotspot

ndhC-trnV-UAC

LSC · 51582-52664 · 1083 bp

Back to Bletilla

Pi 0.207

MarkerSeek score 77.10

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1083
variable_sites: 229
pi: 0.207
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.60
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TGCCAAGATAGGAATAACGCT	AGAAGGTCTACGGTTCGAGT	42.9	50.0	57.2	57.8	733-1188 bp	1.0	75.7
ndhC-trnV-UAC_p2	TGCCAAGATAGGAATAACGCTT	AGAAGGTCTACGGTTCGAGT	40.9	50.0	57.8	57.8	733-1188 bp	1.0	73.2
ndhC-trnV-UAC_p3	TGCCAAGATAGGAATAACGCT	GAAGGTCTACGGTTCGAGTC	42.9	55.0	57.2	57.5	732-1187 bp	1.0	73.0
ndhC-trnV-UAC_p4	TGCCAAGATAGGAATAACGCT	GTTCGAGTCCGTATAGCCCT	42.9	55.0	57.2	58.7	721-1176 bp	1.0	71.8
ndhC-trnV-UAC_p5	ATGCCAAGATAGGAATAACGCT	AGAAGGTCTACGGTTCGAGT	40.9	50.0	57.6	57.8	734-1189 bp	1.0	71.6