Pi
0.0508
MarkerSeek score
82.70
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | TAAAACCTGGCCTGTTGACC | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.0 | 58.1 | 1244-1306 bp | 1.0 | 88.2 |
| trnT-UGU-trnL-UAA_p2 | TAAAACCTGGCCTGTTGACC | GAGTCTCTGCACCTATCCCT | 50.0 | 55.0 | 58.0 | 57.9 | 1398-1460 bp | 1.0 | 87.7 |
| trnT-UGU-trnL-UAA_p3 | AGAAAAAGAGCCGGCTATCG | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 659-714 bp | 0.75 | 76.9 |
| trnT-UGU-trnL-UAA_p4 | TAAAACCTGGCCTGTTGACC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.0 | 58.0 | 1236-1298 bp | 0.75 | 74.5 |
| trnT-UGU-trnL-UAA_p5 | AATTGTCGCTCTGTCAGACC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.9 | 58.0 | 1181-1243 bp | 0.75 | 74.1 |