Hotspot

psaA-ycf3

LSC · 43055-43639 · 585 bp

Back to Bletilla

Pi 0.0504

MarkerSeek score 97.80

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 585
variable_sites: 55
pi: 0.0504
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 97.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	ATAGCGCTTACTCCAGGGAA	50.0	50.0	57.9	58.2	733-790 bp	1.0	87.3
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	TAGCGCTTACTCCAGGGAAT	50.0	50.0	57.9	58.2	732-789 bp	1.0	87.3
psaA-ycf3_p3	CCCATTCCTCGAAAGACGTT	GTTCGATCAAGCTGCTGAGT	50.0	50.0	57.9	58.3	767-824 bp	1.0	86.5
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	GCTATAGCGCTTACTCCAGG	50.0	55.0	57.9	57.6	736-793 bp	1.0	86.1
psaA-ycf3_p5	CCCATTCCTCGAAAGACGTT	CTTGGTTCGATCAAGCTGCT	50.0	50.0	57.9	58.6	771-828 bp	1.0	84.3