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trnC-GCA-petN

LSC · 28752-29592 · 841 bp

Back to Bletilla

Pi 0.0704

MarkerSeek score 84.60

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 841
variable_sites: 98
pi: 0.0704
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	CCAGTTCGAATCTGGGTGTC	GCTCTAAAGCCCACTCCTTC	55.0	55.0	58.3	58.0	950-967 bp	0.5	65.3
trnC-GCA-petN_p2	TAAGCGACGCGTATTCTGAG	GCTCTAAAGCCCACTCCTTC	50.0	55.0	57.9	58.0	1354 bp	0.25	48.9
trnC-GCA-petN_p3	TAAGCGACGCGTATTCTGAG	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.9	58.2	1288 bp	0.25	48.0
trnC-GCA-petN_p4	TAAGCGACGCGTATTCTGAG	CCTTCCCCATACTACAAGCG	50.0	55.0	57.9	57.8	1339 bp	0.25	47.3
trnC-GCA-petN_p5	GCGACGCGTATTCTGAGATA	GCTCTAAAGCCCACTCCTTC	50.0	55.0	57.7	58.0	1351 bp	0.25	47.3