Hotspot

ndhA

SSC · 120242-122405 · 2164 bp

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Pi 0.00231

MarkerSeek score 58.70

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2164
variable_sites: 5
pi: 0.00231
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CCCGGTTATCATAGGAAGCA	TGACGATACTCGGTAGCATAGA	50.0	45.5	56.8	58.0	2319-2320 bp	1.0	54.4
ndhA_p2	ACCCGGTTATCATAGGAAGC	TGACGATACTCGGTAGCATAGA	50.0	45.5	56.8	58.0	2320-2321 bp	1.0	54.4
ndhA_p3	CCCGGTTATCATAGGAAGCA	ATGACGATACTCGGTAGCAT	50.0	45.0	56.8	55.9	2320-2321 bp	1.0	54.0
ndhA_p4	ACCCGGTTATCATAGGAAGC	ATGACGATACTCGGTAGCAT	50.0	45.0	56.8	55.9	2321-2322 bp	1.0	54.0
ndhA_p5	AACCCGGTTATCATAGGAAGC	TGACGATACTCGGTAGCATAGA	47.6	45.5	57.5	58.0	2321-2322 bp	1.0	52.3