Hotspot

ycf10/cemA-petA

LSC · 61246-61475 · 230 bp

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Pi 0.013

MarkerSeek score 60.00

Primer available yes

Label ycf10/cemA-petA

Feature Metadata

label_name: ycf10/cemA-petA
feature_type: igs
region: LSC
length_bp: 230
variable_sites: 3
pi: 0.013
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf10/cemA-petA_p1	CGTGTATCTCCGTCACTTGT	GGAGCGAGTAATCTCTTCCC	50.0	55.0	57.4	57.2	329-335 bp	1.0	69.2
ycf10/cemA-petA_p2	CGTGTATCTCCGTCACTTGT	ATGCACTTGAAATGGATGCC	50.0	45.0	57.4	57.0	384-390 bp	1.0	67.8
ycf10/cemA-petA_p3	CGTGTATCTCCGTCACTTGT	AGTGAGACAGATATGGAGCGA	50.0	47.6	57.4	58.1	343-349 bp	1.0	67.1
ycf10/cemA-petA_p4	CGTGTATCTCCGTCACTTGT	TGAGACAGATATGGAGCGAGT	50.0	47.6	57.4	58.1	341-347 bp	1.0	67.1
ycf10/cemA-petA_p5	TCGTGTATCTCCGTCACTTG	GGAGCGAGTAATCTCTTCCC	50.0	55.0	57.1	57.2	330-336 bp	1.0	67.0