Hotspot

ndhC-trnV

LSC · 50243-51090 · 848 bp

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Pi 0.00237

MarkerSeek score 60.70

Primer available yes

Label ndhC-trnV

Feature Metadata

label_name: ndhC-trnV
feature_type: igs
region: LSC
length_bp: 848
variable_sites: 2
pi: 0.00237
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV_p1	TGCCAAAACAGGAATAGCACT	GTTCGAGTCCGTATAGCCCT	42.9	55.0	58.1	58.7	936-941 bp	1.0	66.1
ndhC-trnV_p2	TGCCAAAACAGGAATAGCACT	TTCGAGTCCGTATAGCCCTA	42.9	50.0	58.1	57.1	935-940 bp	1.0	64.1
ndhC-trnV_p3	TGCCAAAACAGGAATAGCAC	GTTCGAGTCCGTATAGCCCT	45.0	55.0	56.6	58.7	936-941 bp	1.0	64.0
ndhC-trnV_p4	TGCCAAAACAGGAATAGCACT	TCGAACTCTTTTACCGAGCAG	42.9	47.6	58.1	58.1	964-969 bp	1.0	63.1
ndhC-trnV_p5	TGCCAAAACAGGAATAGCACT	TCGAACTCTTTTACCGAGCA	42.9	45.0	58.1	56.9	964-969 bp	1.0	62.8