Hotspot

trnL-trnF

LSC · 47273-47916 · 644 bp

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Pi 0.0161

MarkerSeek score 71.10

Primer available yes

Label trnL-trnF

Feature Metadata

label_name: trnL-trnF
feature_type: igs
region: LSC
length_bp: 644
variable_sites: 10
pi: 0.0161
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-trnF_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	713-734 bp	1.0	79.2
trnL-trnF_p2	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	710-731 bp	1.0	76.0
trnL-trnF_p3	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	50.0	50.0	57.0	58.1	702-723 bp	1.0	75.4
trnL-trnF_p4	AATCGTGAGGGTTCAAGTCC	TACCAACTGAGCTATCCCGG	50.0	55.0	57.5	58.6	675-696 bp	1.0	75.1
trnL-trnF_p5	AATCGTGAGGGTTCAAGTCC	TGAACTGGTGACACGAGGAT	50.0	50.0	57.5	58.7	711-732 bp	1.0	74.3