Hotspot

trnT-trnL

LSC · 45919-46688 · 770 bp

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Pi 0.0039

MarkerSeek score 61.10

Primer available yes

Label trnT-trnL

Feature Metadata

label_name: trnT-trnL
feature_type: igs
region: LSC
length_bp: 770
variable_sites: 3
pi: 0.0039
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-trnL_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCAATTCC	55.0	55.0	57.9	58.2	831-832 bp	1.0	78.1
trnT-trnL_p2	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.9	58.2	834-835 bp	1.0	77.4
trnT-trnL_p3	AAAATAGGGAAAAGCCGGCT	CCGTAGCGTCTACCAATTCC	45.0	55.0	57.8	58.2	886-887 bp	1.0	77.3
trnT-trnL_p4	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCAATTCC	50.0	55.0	57.8	58.2	863-864 bp	1.0	77.2
trnT-trnL_p5	AAAATAGGGAAAAGCCGGCT	AGTCCGTAGCGTCTACCAAT	45.0	50.0	57.8	58.2	889-890 bp	1.0	76.6