Hotspot

rps4-trnT

LSC · 45450-45843 · 394 bp

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Pi 0.00254

MarkerSeek score 51.40

Primer available yes

Label rps4-trnT

Feature Metadata

label_name: rps4-trnT
feature_type: igs
region: LSC
length_bp: 394
variable_sites: 1
pi: 0.00254
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT_p1	CGGAGCGTGATTGGTTTCTA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.0	57.9	573 bp	1.0	79.0
rps4-trnT_p2	CGGAGCGTGATTGGTTTCTA	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.0	57.8	541 bp	1.0	78.2
rps4-trnT_p3	TTACGGAGCGTGATTGGTTT	CTCAGAGGTTAGAGCATCGC	45.0	55.0	57.8	57.9	576 bp	1.0	77.7
rps4-trnT_p4	TTTACGGAGCGTGATTGGTT	CTCAGAGGTTAGAGCATCGC	45.0	55.0	57.8	57.9	577 bp	1.0	77.7
rps4-trnT_p5	TTTTACGGAGCGTGATTGGT	CTCAGAGGTTAGAGCATCGC	45.0	55.0	57.8	57.9	578 bp	1.0	77.7