Hotspot

ycf6-psbM

LSC · 28064-28620 · 557 bp

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Pi 0

MarkerSeek score 61.20

Primer available yes

Label ycf6-psbM

Feature Metadata

label_name: ycf6-psbM
feature_type: igs
region: LSC
length_bp: 557
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf6-psbM_p1	TCGTAGTGTGGGGAAGAAGT	GCACTCTTCATTCTCGTTCCT	50.0	47.6	58.0	58.0	639-658 bp	1.0	74.0
ycf6-psbM_p2	CGTAGTGTGGGGAAGAAGTG	GCACTCTTCATTCTCGTTCCT	55.0	47.6	57.9	58.0	638-657 bp	1.0	73.4
ycf6-psbM_p3	TCGTAGTGTGGGGAAGAAGT	ACTGCACTCTTCATTCTCGT	50.0	45.0	58.0	56.9	642-661 bp	1.0	73.3
ycf6-psbM_p4	CGTAGTGTGGGGAAGAAGTG	ACTGCACTCTTCATTCTCGT	55.0	45.0	57.9	56.9	641-660 bp	1.0	72.8
ycf6-psbM_p5	CTCTCGTAGTGTGGGGAAGA	GCACTCTTCATTCTCGTTCCT	55.0	47.6	58.2	58.0	642-661 bp	1.0	72.7