Hotspot

trnC-ycf6

LSC · 27313-27973 · 661 bp

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Pi 0.00308

MarkerSeek score 62.10

Primer available yes

Label trnC-ycf6

Feature Metadata

label_name: trnC-ycf6
feature_type: igs
region: LSC
length_bp: 661
variable_sites: 2
pi: 0.00308
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-ycf6_p1	GTTTCTATGGTTTTGGCGGC	ACTTCTTCCCCACACTACGA	50.0	50.0	58.0	58.0	823-828 bp	1.0	81.1
trnC-ycf6_p2	TTTCCCCAGTTCAAATCCGG	ACTTCTTCCCCACACTACGA	50.0	50.0	58.1	58.0	767-772 bp	1.0	80.7
trnC-ycf6_p3	GTTTCTATGGTTTTGGCGGC	CACTTCTTCCCCACACTACG	50.0	55.0	58.0	57.9	824-829 bp	1.0	80.5
trnC-ycf6_p4	TTTCCCCAGTTCAAATCCGG	CACTTCTTCCCCACACTACG	50.0	55.0	58.1	57.9	768-773 bp	1.0	80.1
trnC-ycf6_p5	TTCTATGGTTTTGGCGGCAT	ACTTCTTCCCCACACTACGA	45.0	50.0	58.2	58.0	821-826 bp	1.0	80.0