Hotspot

atpF

LSC · 11670-13101 · 1432 bp

Back to Blastus

Pi 0.00591

MarkerSeek score 62.70

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1432
variable_sites: 23
pi: 0.00591
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CGTCGGCTTTAATGGTTACC	TCTGTAAAAGAAAGGGGATTGT	50.0	36.4	56.9	55.3	1462-1528 bp	1.0	44.9
atpF_p2	TCGTCGGCTTTAATGGTTACC	TCTGTAAAAGAAAGGGGATTGT	47.6	36.4	58.4	55.3	1463-1529 bp	1.0	44.9
atpF_p3	CGTCGGCTTTAATGGTTACC	ATCTGTAAAAGAAAGGGGATTGT	50.0	34.8	56.9	55.8	1463-1529 bp	1.0	44.9
atpF_p4	CGTCGGCTTTAATGGTTACCA	TCTGTAAAAGAAAGGGGATTGT	47.6	36.4	58.7	55.3	1462-1528 bp	1.0	44.9
atpF_p5	CGTCGGCTTTAATGGTTACC	CTGTAAAAGAAAGGGGATTGT	50.0	38.1	56.9	53.7	1461-1527 bp	1.0	44.9