Hotspot

rps15-trnN-GUU

SSC · 125179-131322 · 6144 bp

Back to Blastus

Pi 0.00717

MarkerSeek score 47.50

Primer available yes

Label rps15-trnN-GUU

Feature Metadata

label_name: rps15-trnN-GUU
feature_type: igs
region: SSC
length_bp: 6144
variable_sites: 121
pi: 0.00717
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-trnN-GUU_p1	TCAAAAATCCATTTAACCGATCAGT	ATCCCCCGTTGAAAATTCGT	32.0	45.0	57.5	57.8	308-321 bp	1.0	47.7
rps15-trnN-GUU_p2	TCAAAAATCCATTTAACCGATCAGT	TATCGAACCTGTGCGTTTGT	32.0	45.0	57.5	57.8	813-826 bp	1.0	44.0
rps15-trnN-GUU_p3	CAAAAATCCATTTAACCGATCAGT	TATCGAACCTGTGCGTTTGT	33.3	45.0	56.2	57.8	812-825 bp	1.0	44.0
rps15-trnN-GUU_p4	AATCCATTTAACCGATCAGT	TATCGAACCTGTGCGTTTGT	35.0	45.0	52.2	57.8	808-821 bp	1.0	44.0
rps15-trnN-GUU_p5	TCAAAAATCCATTTAACCGATCAGT	CGAACCTGTGCGTTTGTCTA	32.0	50.0	57.5	58.5	817-823 bp	0.375	16.2