Hotspot

ndhA-ndhH

SSC · 123606-123606 · 1 bp

Back to Blastus

Pi 0.25
MarkerSeek score 63.70
Primer available yes
Label ndhA-ndhH

Feature Metadata

label_name
ndhA-ndhH
feature_type
igs
region
SSC
length_bp
1
variable_sites
1
pi
0.25
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhA-ndhH_p1 AAAGCATCCCTATGATCCCA CCCCCGGTTTTATCAATTTGC 45.0 47.6 55.9 58.1 208 bp 1.0 55.1
ndhA-ndhH_p2 AAAAGCATCCCTATGATCCCA CCCCCGGTTTTATCAATTTGC 42.9 47.6 56.7 58.1 209 bp 1.0 53.5
ndhA-ndhH_p3 AAGCATCCCTATGATCCCAT CCCCCGGTTTTATCAATTTGC 45.0 47.6 55.7 58.1 207 bp 1.0 53.2
ndhA-ndhH_p4 AAAGCATCCCTATGATCCCA TGGGAGAAGTTGATCGTTGA 45.0 45.0 55.9 56.5 112 bp 1.0 52.1
ndhA-ndhH_p5 AAAGCATCCCTATGATCCCAT CCCCCGGTTTTATCAATTTGC 42.9 47.6 56.5 58.1 208 bp 1.0 51.7