Hotspot

ndhD-psaC

SSC · 118765-118907 · 143 bp

Back to Blastus

Pi 0.036

MarkerSeek score 68.40

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 143
variable_sites: 11
pi: 0.036
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	TGTTAACCAAGGAAAATAATTCGT	AAACAACTCGAAGCATGGGT	29.2	45.0	55.3	58.0	196-200 bp	1.0	45.6
ndhD-psaC_p2	TTGTTAACCAAGGAAAATAATTCGT	AAACAACTCGAAGCATGGGT	28.0	45.0	56.0	58.0	197-201 bp	1.0	45.6
ndhD-psaC_p3	TGTTAACCAAGGAAAATAATTCGT	GGCATGAAACAACTCGAAGC	29.2	50.0	55.3	58.0	202-206 bp	1.0	45.5
ndhD-psaC_p4	TTGTTAACCAAGGAAAATAATTCGT	GGCATGAAACAACTCGAAGC	28.0	50.0	56.0	58.0	203-207 bp	1.0	45.5
ndhD-psaC_p5	TGTTAACCAAGGAAAATAATTCGT	AGAGATGTGAATCCGCTTGC	29.2	50.0	55.3	58.3	256-260 bp	1.0	44.3