Hotspot

ccsA-ndhD

SSC · 116935-117252 · 318 bp

Back to Blastus

Pi 0.0267

MarkerSeek score 61.90

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 318
variable_sites: 21
pi: 0.0267
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ATGCAGCTCTTTTATGCGGA	TCAATGGCTTCCCTTGCATT	45.0	45.0	58.0	58.1	1194-1213 bp	1.0	81.6
ccsA-ndhD_p2	TGCAGCTCTTTTATGCGGAT	TCAATGGCTTCCCTTGCATT	45.0	45.0	58.0	58.1	1193-1212 bp	1.0	81.6
ccsA-ndhD_p3	TGCAGCTCTTTTATGCGGAT	TTTTGATTCTGGGCCGAGAG	45.0	50.0	58.0	57.9	975-994 bp	1.0	81.6
ccsA-ndhD_p4	TTAGTACCCGCGCTTCAATC	TCAATGGCTTCCCTTGCATT	50.0	45.0	58.1	58.1	1255-1274 bp	1.0	81.3
ccsA-ndhD_p5	TGCAGCTCTTTTATGCGGAT	CTCGACAACATGGGTGGAAT	45.0	50.0	58.0	57.9	1256-1275 bp	1.0	81.0