Hotspot

rps18-rpl20

LSC · 69674-70013 · 340 bp

Back to Blastus

Pi 0.0162

MarkerSeek score 55.70

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 340
variable_sites: 17
pi: 0.0162
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	GACTGCCAGAGCTAATGGTC	GTCTTTATATGATTTCTAATTCGATCA	55.0	25.9	58.1	54.1	395-418 bp	1.0	47.3
rps18-rpl20_p2	GACTGCCAGAGCTAATGGTC	TGTCTTTATATGATTTCTAATTCGATC	55.0	25.9	58.1	54.1	396-419 bp	1.0	47.3
rps18-rpl20_p3	ATCGACTGCCAGAGCTAATG	GTCTTTATATGATTTCTAATTCGATCA	50.0	25.9	57.5	54.1	398-421 bp	1.0	47.3
rps18-rpl20_p4	ATCGACTGCCAGAGCTAATG	TGTCTTTATATGATTTCTAATTCGATC	50.0	25.9	57.5	54.1	399-422 bp	1.0	47.3
rps18-rpl20_p5	GACTGCCAGAGCTAATGGTC	GTCTTTATATGATTTCTAATTCGATC	55.0	26.9	58.1	52.6	395-418 bp	1.0	47.3