Hotspot

trnS-UGA-psbZ

LSC · 36529-36905 · 377 bp

Back to Blastus

Pi 0.0113

MarkerSeek score 61.00

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 377
variable_sites: 15
pi: 0.0113
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	TTTTCAAGACCGGAGCCATC	GGAGAAGCAAAAACAACGGG	50.0	50.0	58.2	57.9	499-506 bp	1.0	81.0
trnS-UGA-psbZ_p2	TTTTCAAGACCGGAGCCATC	TTGTTACTTGACCAGCCGTC	50.0	50.0	58.2	58.1	520-527 bp	1.0	80.9
trnS-UGA-psbZ_p3	AGCAAAAGGAGAGAGAGGGA	GGAGAAGCAAAAACAACGGG	50.0	50.0	57.7	57.9	558-565 bp	1.0	79.4
trnS-UGA-psbZ_p4	AGCAAAAGGAGAGAGAGGGA	TTGTTACTTGACCAGCCGTC	50.0	50.0	57.7	58.1	579-586 bp	1.0	79.3
trnS-UGA-psbZ_p5	TTTTCAAGACCGGAGCCATC	TTTGTTACTTGACCAGCCGT	50.0	45.0	58.2	57.7	521-528 bp	1.0	79.2