Hotspot

psbM-trnD-GUC

LSC · 29990-30707 · 718 bp

Back to Blastus

Pi 0.00746
MarkerSeek score 63.20
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
718
variable_sites
15
pi
0.00746
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 TCACTTTGACTCACGGTTTT TCAATTGGTTAGAGCACCGC 40.0 50.0 55.4 58.6 878-895 bp 1.0 57.9
psbM-trnD-GUC_p2 TCACTTTGACTCACGGTTTTT TCAATTGGTTAGAGCACCGC 38.1 50.0 56.2 58.6 878-895 bp 1.0 56.2
psbM-trnD-GUC_p3 AATCACTTTGACTCACGGTT TCAATTGGTTAGAGCACCGC 40.0 50.0 55.2 58.6 880-897 bp 1.0 56.0
psbM-trnD-GUC_p4 ATCACTTTGACTCACGGTTT TCAATTGGTTAGAGCACCGC 40.0 50.0 55.2 58.6 879-896 bp 1.0 56.0
psbM-trnD-GUC_p5 AGAATGAAGAGTGCAGTAGC TCAATTGGTTAGAGCACCGC 45.0 50.0 55.2 58.6 824-841 bp 1.0 55.9