Hotspot

trnR-UCU-atpA

LSC · 9797-10105 · 309 bp

Back to Blastus

Pi 0.0142
MarkerSeek score 63.10
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
309
variable_sites
13
pi
0.0142
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 AACACCAAACCAGAAGCCAA CATTCACCGAGGAAGCAGAA 45.0 50.0 58.1 57.9 495-550 bp 1.0 80.4
trnR-UCU-atpA_p2 ACACCAAACCAGAAGCCAAA CATTCACCGAGGAAGCAGAA 45.0 50.0 58.1 57.9 494-549 bp 1.0 80.4
trnR-UCU-atpA_p3 AACACCAAACCAGAAGCCAA AAGACATTCACCGAGGAAGC 45.0 50.0 58.1 57.9 499-554 bp 1.0 80.4
trnR-UCU-atpA_p4 ACACCAAACCAGAAGCCAAA AAGACATTCACCGAGGAAGC 45.0 50.0 58.1 57.9 498-553 bp 1.0 80.4
trnR-UCU-atpA_p5 AGAACACCAAACCAGAAGCC CATTCACCGAGGAAGCAGAA 50.0 50.0 58.3 57.9 497-552 bp 1.0 79.1