Hotspot

trnH-GUG-psbA

LSC · 76-274 · 199 bp

Back to Blastus

Pi 0.0336

MarkerSeek score 73.40

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 199
variable_sites: 22
pi: 0.0336
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	AGCTGCTGTTGAAGTTCCAT	55.0	45.0	58.1	57.7	253-303 bp	1.0	86.0
trnH-GUG-psbA_p2	AATCCACTGCCTTGATCCAC	AGCTGCTGTTGAAGTTCCAT	50.0	45.0	57.9	57.7	266-316 bp	1.0	85.6
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	AGCTGCTGTTGAAGTTCCAT	50.0	45.0	57.9	57.7	267-317 bp	1.0	85.5
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	AGCTGCTGTTGAAGTTCCAT	50.0	45.0	57.9	57.7	268-318 bp	1.0	85.5
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	GACCTAGCTGCTGTTGAAGT	55.0	50.0	58.1	57.5	258-308 bp	1.0	84.4