Hotspot

psbA

LSC · 265-1326 · 1062 bp

Back to Bistorta

Pi 0.0079

MarkerSeek score 43.70

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 22
pi: 0.0079
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	AGGAGCAATACCAACCCTCT	CGCGGAGTTTTAGGTGTGTA	50.0	50.0	58.0	57.9	1540 bp	0.222222	48.3
psbA_p2	GAGCAATACCAACCCTCTCG	CGCGGAGTTTTAGGTGTGTA	55.0	50.0	58.1	57.9	1538 bp	0.222222	48.0
psbA_p3	AGGAGCAATACCAACCCTCT	CATCTAGGAAAGTCGCGGAG	50.0	55.0	58.0	57.9	1553 bp	0.222222	47.8
psbA_p4	GAGCAATACCAACCCTCTCG	CATCTAGGAAAGTCGCGGAG	55.0	55.0	58.1	57.9	1551 bp	0.222222	47.6
psbA_p5	AGGAGCAATACCAACCCTCT	GGACCTCGAGTTCTACTGGT	50.0	55.0	58.0	58.2	1320 bp	0.222222	47.5